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For example, in one embodiment, spray-dried Pichia cells have spinach glycolate oxidase and Sacharromyces catalase T.
However, the invention is not limited to particular microbes and/or particular enzyme catalyzed reactions, as it is contemplated that microbes generally, whether recombinant or nonrecombinant, may be spray-dried and result in a source of stable enzyme(s) for biocatalysis.
b) spray drying the recombinant yeast cell suspension in the absence of a permeabilization agent at a temperature up to 150° C. Electrochemical manufacture involves either the reduction of oxalic acid or the anodic oxidation of glyoxal to form glyoxylic acid whereas chemical oxidation generally involves the oxidation of glyoxal in the presence of a strong acid such as HNO. Spray-drying of microbial cells, such as Pichia, Saccharomyces or E.
effective to yield a spray-dried recombinant yeast cell preparation suitable for biocatalysis, wherein the spray-dried cells are porous but retain an enzyme active in the biocatalysis. A consequence of these commercial processes is the production of waste streams containing various toxic acids and heavy metals. Chem., 15 (1949), reported an enzyme, extracted from tobacco leaves, which catalyzed the oxidation of glycolic acid to formic acid and COvia the intermediate formation of glyoxylic acid. Chem., 201 (1962), reported that the formation of formic acid and CO. coli, renders the cells suitable for biocatalysis via opening of the cell wall or cell membrane in the absence of permeabilization agents allowing for substrates and products to freely move in and out of the cells.
An oligonucleotide typically contains a sequence of about two to about one hundred bases.
Products of the reaction, for instance, pyruvate, glyoxylate, keto acids or chiral hydroxyl acids, are then isolated from the aqueous medium. * After lysing the cells by French Press, the lysate was centrifuged at 25,800×g for 10 minutes, and the resulting supernatant (about 10 m L) was saved as the cell extract.The method includes combining a spray-dried preparation of the invention and an aqueous solution comprising a substrate for an enzyme present in the spray-dried preparation under conditions that yield one or more products of the enzyme catalyzed reaction.In one embodiment, the product is pyruvic acid or its salts, which are useful in food, pharmaceutical and agrochemical products, e.g., in dietary supplements, cosmetics or oxygenated solvents, and production of amino acids, hydrazone, indoxocarb and indole-3-pyruvic acid.The methods for preparing the spray-dried microbial cell biocatalyst surprisingly do not require detergent or other membrane permeabilization agent treatment, e.g., do not require a cationic surfactant, such as benzylalkonium chloride (BAC), treatment, or enzyme stabilizing agents such as sucrose, trehalose, thiols or other polyols.Further provided is a method to prepare products of an enzymatic reaction.